Cellular Signalling

We also employ in-cell NMR spectroscopy to study entire cellular signaling networks.

Using peptide-based Kinase Activity Reporters (KARs) and in-cell NMR readouts we 'measure' dynamic changes of cellular kinase activities under different physiological and pathophysiological conditions. 

KARs enable multiplexed readouts of cellular kinase activities in qualitative and quantitative terms. In addition, they allow us to monitor kinase activities- and dynamic changes thereof- in real time.

We study global kinase activity profiles of different human cancer cell lines and screen for drugs that selectively modulate individual signalling pathways.

Besides protein phosphorylation and intracellular kinase activities, we also design NMR reporters to profile histone acetyl-transferase (HAT) and deacetylase (HDAC) activities.

All of our in-cell NMR reporters are designed to be used in parallel. This enables the simultaneous detection of different types of cellular PTM activities in a single NMR experiment.