Opposing effects of Elk-1 multisite

phosphorylation shape its response to ERK activation. 

Mylona A, Theillet FX, Foster C, Cheng TM, Miralles F,

Bates PA, Selenko P, Treisman R. 

Science. 2016 Oct;354(6309):233-237

In-cell protein structures from 2D NMR experiments. 

Muntener T, Hausssinger D, Selenko P, Theillet FX. 

J Phys Chem Lett. 2016 Jul;7(14):2821-5

Structural disorder of monomeric alpha-synuclein persists in mammalian cells. 

Theillet FX, Binolfi A, Beata B, Martorana A, Rose HM,

Stuiver M, Verzini S, Lorenz D, van Rossum M,

Goldfarb D, Selenko P. 

Nature. 2016 Feb;530(7588):45-50

Intracellular repair of oxidation-damaged alpha-synuclein fails to target C-terminal modification sites. 

Binolfi A, Limatola A, Verzini S, Kosten J, Theillet FX, Rose HM, Bekei B, Stuiver M, van Rossum M, Selenko P. 

Nat Commun. 2016 Jan;25(7):10251

Modulations of DNA contacts by linker histones

and post-translational modifications determine

the mobility and modifiability of nucleosomal H3 tails. 

Stutzer A, Liokatis S, Kiesel A, Schwarzer D, Sprangers R,

Soding J, Selenko P, Fischle W. 

Mol Cell. 2016 Jan;61(2):247-59

Thermodynamics of protein destabilization

in live cells. 

Danielsson J, Mu X, Lang L, Wang H, Binolfi A, Theillet FX,

Bekei B, Logan DT, Selenko P, Wennerstrom H, Oliveberg M. 

Proc Natl Acad Sci USA. 2015 Oct;112(40):12402-7

A multiplexed NMR-reporter approach

to measure cellular kinase and phosphatase activities in real-time. 

Thongwichian R, Kosten J, Benary U, Rose HM, Stuiver M,

Theillet FX, Dose A, Koch B, Yokoyama H, Schwarzer D,

Wolf J, Selenko P. 

J Am Chem Soc. 2015 May;137(20):6468-6471

Copper binding to the N-terminally acetylated, naturally occurring form of alpha-synuclein

induces local helical folding. 

Miotto MC, Valiente-Gabioud AA, Rosetti G, Zweckstetter M, Carloni P, Selenko P, Griesinger C, Binolfi A, Fernandez CO.

J Am Chem Soc. 2015 May;137(20):6444-6447

Real-time monitoring of biological activities in complex physiological environments.  

Smith MJ, Marshall CB, Theillet FX, Binolfi A, Selenko P,

Ikura M.

Curr Opin Struct Biol. 2015 Jun;32:39-47

Disorder and residual helicity alter p53-Mdm2

binding affinity and signaling in cells. 

Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM,

Powell AT, Wu H, Manieri W, Dieterich C, Selenko P,

Loewer A, Daughdrill G.

Nat Chem Biol. 2014 Dec;10(12):1000-2

Efficient modification of alpha-synuclein serine 129

by protein kinase CK1 requires phosphorylation of tyrosine 125 as a priming event. 

Kosten J, Binolfi A, Stuiver M, Verzini S, Theillet FX, Bekei B,

van Rossum M, Selenko P.

ACS Chem Neurosci. 2014 Oct;(Epub ahead of print) 

Physicochemical properties of cells and their effects

on intrinsically disordered proteins (IDPs). 

Theillet FX, Binolfi A, Fremberg-Kesner T, Hingorani K, Sarkar M, Kyne C, Crowley PB, Gieraasch L, Pielak GJ, Elcock AH, Gershenson A, Selenko P.

Chem Rev. 2014 Jul;114(13):6661-714 

Live Cell NMR. 

Freedberg D, Selenko P.

Annu Rev Biophys. 2014 Jun;43:171-92 

Site-specific mapping and time-resolved monitoring

of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts. 

Theillet FX, Rose HM, Liokatis S, Binolfi A, Thongwichian R, Stuiver M, Selenko P.

Nat Protoc. 2013 Jul;8(7):1416-32 

Quantitative NMR analysis of Erk activity and

inhibition by U0126 in a panel of patient-derived colorectal cancer cell lines.

Rose HM, Stuiver M, Thongwichian R, Theillet FX, Feller SM, Selenko P.

Biochim Biophys Acta, 2013 Jul;1834(7):1396-401 

Asymmetrically modified nucleosomes expand the histone code.

van Rossum B, Fischle W, Selenko P.

Nat Struct Mol Biol. 2012 Nov;19(11):1064-6. 

Cell signaling, post-translational protein modifications and NMR spectroscopy.

Theillet FX, Smet-Noca C, Liokatis S, Thongwichian R, Kosten J, Yoon MK, Kriwacki RW, Landrieu I, Lippens G, Selenko P.

J Biomol NMR. 2012 Nov;54(3):217-36. 

Bacterial in-cell NMR of human alpha-synuclein: a disordered monomer by nature?

Binolfi A, Theillet FX, Selenko P.

Biochem Soc Trans. 2012 Oct;40(5):950-4. 

Phosphorylation of histone H3 Ser 10 establishes a hierarchy for subsequent intramolecular modification events.

Liokatis S, Stutzer A, Elsasser S, Theillet FX, Klingberg R, van Rossum B, Schwarzer D, Allis CD, Fischle W, Selenko P.

Nat Struct Mol Biol. 2012 Aug;19(8):819-23. 

In-cell NMR in mammalian cells: Part 3.

Bekei B, Rose HM, Herzig M, Stephanowitz H, Krause E, Selenko P.

Methods Mol Biol. 2012 ;895:67-83. 

In-cell NMR in mammalian cells: Part 2.

Bekei B, Rose HM, Herzig M, Selenko P.

Methods Mol Biol. 2012 ;895:55-66. 

In-cell NMR in mammalian cells: Part 1.

Bekei B, Rose HM, Herzig M, Dose A, Schwarzer D, Selenko P.

Methods Mol Biol. 2012 ;895:43-54. 

In-cell NMR in Xenopus laevis oocytes.

Thongwichian R, Selenko P.

Methods Mol Biol. 2012 ;895:33-41. 

Site-specific mapping and time-resolved monitoring of lysine methylation by high-resolution NMR spectroscopy.

Theillet FX, Liokatis S, Jost JO, Bekei B, Rose HM, Binolfi A, Schwarzer D, Selenko P.

J Am Chem Soc. 2012 May 9;134(18):7616-9. 

Paramagnetic relaxation enhancement to improve sensitivity of fast NMR methods: application to intrinsically disordered proteins.

Theillet FX, Binolfi A, Liokatis S, Verzini S, Selenko P.

J Biomol NMR. 2011 Dec;51(4):487-95. 

Structure of the VP16 transactivator target in the mediator complex.

Milbradt AG, Kulkarni M, Yi T, Takeuchi K, Sun ZY, Luna RE,

Selenko P, Näär AM, Wagner G.

Nat Struct Mol Biol. 2011 Apr;18(4):410-5. Epub 2011 Mar 6.

NMR profiling of histone deacetylase and acetyl-transferase activities in real time.

Dose A, Liokatis S, Theillet FX, Selenko P, Schwarzer D.

ACS Chem Biol. 2011 Mar 7. [Epub ahead of print]

Simultaneous detection of protein phosphorylation

and acetylation by high-resolution NMR

spectroscopy.

Liokatis S, Dose A, Schwarzer D, Selenko P.

J Am Chem Soc. 2010 Oct 27;132(42):14704-5.

Cellular Structural Biology.

Ito Y, Selenko P.

Curr Opin Struct Biol. 2010 Oct;20(5):640-8.

In situ observation of protein phosphorylation

by high-resolution NMR spectroscopy.

Selenko P, Frueh DP, Elsaesser SJ, Haas W, Gygi SP, Wagner G.

Nat Struct Mol Biol. 2008 Mar;15(3):321-9. Epub 2008 Feb 24.

Looking into live cells with in-cell NMR

spectroscopy.

Selenko P, Wagner G.

J Struct Biol. 2007 May;158(2):244-53. Epub 2007 Apr 10.

Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy.

Serber Z, Selenko P, Hänsel R, Reckel S, Löhr F, Ferrell JE Jr, Wagner G, Dötsch V.

Nat Protoc. 2006;1(6):2701-9.

Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes.

Selenko P, Serber Z, Gadea B, Ruderman J, Wagner G.

Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11904-9. Epub 2006 Jul 27.

NMR mapping of protein interactions in living cells.

Selenko P. and Wagner G.

Nat Methods. 2006 Feb;3(2):80-1.